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  • KD/KO Validated

AARS2 Polyclonal antibody

AARS2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 22696-1-AP

Synonyms

AlaRS, Alanyl-tRNA synthetase, Alanyl tRNA synthetase like, Alanine--tRNA ligase, mitochondrial, Alanine tRNA ligase



Tested Applications

Positive WB detected inK-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22696-1-AP targets AARS2 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AARS2 fusion protein Ag18539
Full Name alanyl-tRNA synthetase 2, mitochondrial (putative)
Calculated Molecular Weight 985 aa, 107 kDa
Observed Molecular Weight 105-115 kDa
GenBank Accession NumberBC131728
Gene Symbol AARS2
Gene ID (NCBI) 57505
RRIDAB_11182926
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AARS2(Alanine--tRNA ligase, mitochondrial) is also named as KIAA1270, AARSL, bA444E17.1, AlaRS, AARSL, COXPD8, MTALARS, MT-ALARS and belongs to the class-II aminoacyl-tRNA synthetase family. It catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). It also edits incorrectly charged tRNA(Ala) via its editing domain. The full length 107 kDa protein has a transit peptide with 23 amino acids.

Protocols

Product Specific Protocols
WB protocol for AARS2 antibody 22696-1-APDownload protocol
IHC protocol for AARS2 antibody 22696-1-APDownload protocol
IP protocol for AARS2 antibody 22696-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Res

Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation

Authors - Yunzi Mao
  • KO Validated
ratWB

Cell Stem Cell

A small-molecule cocktail promotes mammalian cardiomyocyte proliferation and heart regeneration.

Authors - Jianyong Du
humanWB

J Hematol Oncol

Integrated proteogenomic characterization of urothelial carcinoma of the bladder.

Authors - Ning Xu
humanWB

Cancer Med

AARS2 as a novel biomarker for prognosis and its molecular characterization in pan-cancer

Authors - Long Liu