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  • Featured Product
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AlaRS Polyclonal antibody

AlaRS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17394-1-AP

Synonyms

AARS, Renal carcinoma antigen NY-REN-42, EC:6.1.1.7, Alanyl-tRNA synthetase, Alanine--tRNA ligase, cytoplasmic

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using AlaRS Polyclonal antibody (17394-1-AP)

    WB analysis of HepG2 using 17394-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17394-1-AP (AlaRS antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using AlaRS Polyclonal antibody (17394-1-AP)

    WB analysis of HepG2 using 17394-1-AP

    WB result of AlaRS antibody (17394-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AlaRS transfected HepG2 cells.

  • Western Blot (WB) analysis of Jurkat cells using AlaRS Polyclonal antibody (17394-1-AP)

    WB analysis of Jurkat using 17394-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17394-1-AP (AlaRS antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using AlaRS Polyclonal antibody (17394-1-AP)

    IP experiment of HepG2 using 17394-1-AP

    IP result of anti-AlaRS (IP:17394-1-AP, 4ug; Detection:17394-1-AP 1:500) with HepG2 cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using AlaRS Polyclonal antibody (17394-1-AP)

    IHC staining of human liver using 17394-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17394-1-AP (AlaRS antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using AlaRS Polyclonal antibody (17394-1-AP)

    IHC staining of human liver using 17394-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17394-1-AP (AlaRS antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AlaRS Polyclonal antibody (17394-1-AP)

    IF Staining of HepG2 using 17394-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AlaRS antibody (17394-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17394-1-AP targets AlaRS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AlaRS fusion protein Ag11294
Full Name alanyl-tRNA synthetase
Calculated Molecular Weight 968 aa, 107 kDa
Observed Molecular Weight 107 kDa
GenBank Accession NumberBC011451
Gene Symbol AlaRS
Gene ID (NCBI) 16
RRIDAB_2219748
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AARS(alanyl-tRNA synthetase) is also named as AlaRS(alanine tRNA ligase 1, cytoplasmic), renal carcinoma antigen NY-REN-42 and belongs to the class-II aminoacyl-tRNA synthetase family. It can interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. AARS consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs(PMID:19661429).

Protocols

Product Specific Protocols
WB protocol for AlaRS antibody 17394-1-APDownload protocol
IHC protocol for AlaRS antibody 17394-1-APDownload protocol
IF protocol for AlaRS antibody 17394-1-APDownload protocol
IP protocol for AlaRS antibody 17394-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Nat Commun

Comprehensive proteogenomic characterization of early duodenal cancer reveals the carcinogenesis tracks of different subtypes

Authors - Lingling Li
View Article