A4GNT Polyclonal antibody
A4GNT Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 22670-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | K-562 cells, mouse pancreas tissue, mouse stomach tissue, rat stomach tissue, L02 cells |
Positive IHC detected in | human stomach cancer tissue, human pancreas cancer tissue, rat stomach tissue, mouse stomach tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
22670-1-AP targets A4GNT in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | A4GNT fusion protein Ag18432 |
Full Name | alpha-1,4-N-acetylglucosaminyltransferase |
Calculated Molecular Weight | 340 aa, 39 kDa |
Observed Molecular Weight | 34-37 kDa |
GenBank Accession Number | BC119639 |
Gene Symbol | A4GNT |
Gene ID (NCBI) | 51146 |
RRID | AB_11183768 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
A4GNT (alpha-1,4-N-acetylglucosaminyltransferase) catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans, forming a unique glycan structure, GlcNAcalpha1-->4Galbeta-->R. The gene is expressed with a bias in certain tissues, particularly in the stomach (RPKM 9.5) and duodenum (RPKM 0.9). Alpha4GnT has been implicated in O-glycan biosynthesis and is thought to have a role in preventing gastric cancer by inhibiting Helicobacter pylori infection and suppressing tumor-promoting inflammation.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for A4GNT antibody 22670-1-AP | Download protocol |
IHC protocol for A4GNT antibody 22670-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |