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A4GALT Polyclonal antibody

A4GALT Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12392-1-AP

Synonyms

EC:2.4.1.228, Alpha4Gal-T1, Alpha-1,4-N-acetylglucosaminyltransferase, Alpha-1,4-galactosyltransferase, A4GALT1

Validation Data Gallery

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  • Western Blot (WB) analysis of RAW 264.7 cells using A4GALT Polyclonal antibody (12392-1-AP)

    WB analysis of RAW 264.7 using 12392-1-AP

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 12392-1-AP (A4GALT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using A4GALT Polyclonal antibody (12392-1-AP)

    WB analysis of K-562 using 12392-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12392-1-AP (A4GALT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using A4GALT Polyclonal antibody (12392-1-AP)

    WB analysis of Raji using 12392-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 12392-1-AP (A4GALT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using A4GALT Polyclonal antibody (12392-1-AP)

    IHC staining of human cervical cancer using 12392-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using A4GALT Polyclonal antibody (12392-1-AP)

    IHC staining of human cervical cancer using 12392-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using A4GALT Polyclonal antibody (12392-1-AP)

    IHC staining of mouse kidney using 12392-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using A4GALT Polyclonal antibody (12392-1-AP)

    IHC staining of mouse kidney using 12392-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using A4GALT Polyclonal antibody (12392-1-AP)

    IF Staining of HeLa using 12392-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using A4GALT antibody (12392-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inRAW 264.7 cells, K-562 cells, Raji cells
Positive IHC detected inhuman cervical cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12392-1-AP targets A4GALT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen A4GALT fusion protein Ag3069
Full Name alpha 1,4-galactosyltransferase
Calculated Molecular Weight 353 aa, 41 kDa
Observed Molecular Weight 50-52 kDa
GenBank Accession NumberBC017068
Gene Symbol A4GALT
Gene ID (NCBI) 53947
RRIDAB_2877853
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for A4GALT antibody 12392-1-APDownload protocol
IHC protocol for A4GALT antibody 12392-1-APDownload protocol
IF protocol for A4GALT antibody 12392-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols