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Alpha 1B-Glycoprotein Monoclonal antibody

Alpha 1B-Glycoprotein Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4D4E5

Cat no : 66260-1-Ig

Synonyms

A1B, A1BG, ABG, alpha 1 B glycoprotein, Alpha 1B glycoprotein, Alpha 1B-Glycoprotein, GAB, HYST2477

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Alpha 1B-Glycoprotein Monoclonal antibody (66260-1-Ig)

    WB analysis of human plasma using 66260-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66260-1-Ig (Alpha 1B-Glycoprotein antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using Alpha 1B-Glycoprotein Monoclonal antibody (66260-1-Ig)

    WB analysis of human heart using 66260-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 66260-1-Ig (alpha-1B-glycoprotein antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using Alpha 1B-Glycoprotein Monoclonal antibody (66260-1-Ig)

    WB analysis of human placenta using 66260-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66260-1-Ig (Alpha 1B-Glycoprotein antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha 1B-Glycoprotein Monoclonal antibody (66260-1-Ig)

    IHC staining of human liver using 66260-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66260-1-Ig (alpha-1B-glycoprotein antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha 1B-Glycoprotein Monoclonal antibody (66260-1-Ig)

    IHC staining of human liver using 66260-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66260-1-Ig (alpha-1B-glycoprotein antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha 1B-Glycoprotein Monoclonal antibody (66260-1-Ig)

    IF Staining of HepG2 using 66260-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66260-1-Ig (alpha-1B-glycoprotein antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman plasma, human heart tissue, human placenta tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66260-1-Ig targets Alpha 1B-Glycoprotein in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Alpha 1B-Glycoprotein fusion protein Ag5414
Full Name alpha-1-B glycoprotein
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberBC035719
Gene Symbol A1BG
Gene ID (NCBI) 1
RRIDAB_2881647
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha-1B-glycoprotein (A1BG) is a plasma protein and is a member of the immunoglobulin superfamily. A1BG contains 474 amino acids and has 5 intrachain disulfide bonds. It has been reported that cysteine-rich secretory protein 3 (CRISP-3) is a specific and high-affinity ligand of A1BG. A1BG-CRISP-3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP3 (PMID: 15461460).

Protocols

Product Specific Protocols
WB protocol for Alpha 1B-Glycoprotein antibody 66260-1-IgDownload protocol
IHC protocol for Alpha 1B-Glycoprotein antibody 66260-1-IgDownload protocol
IF protocol for Alpha 1B-Glycoprotein antibody 66260-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols